Complement fixation test Diagnostic Kit for CBPP

Veterinary product Animal production and health
To prevent the spread of Contagious bovine pleuropneumonia (CBPP), the complement fixation test (CFT) diagnostic kit is a quick solution for a first level of information.
Péripenumonie contagieuse bovine © F. Thiaucourt, Cirad
Péripenumonie contagieuse bovine © F. Thiaucourt, Cirad

Contagious bovine pleuropneumonia © F. Thiaucourt, CIRAD

When to use the CBPP CFT diagnostic kit?

Contagious bovine pleuropneumonia (CBPP) is one of the most important infectious diseases in cattle. Losses can be as high as 80% of the herd, and surviving animals often become chronic carriers, facilitating transmission of the disease.

The CFT diagnostic kit is a reference solution recommended by the World Organization for Animal Health (WOAH) for the diagnosis of this disease at the herd level. The surveillance of herds is essential for the control of the disease due to asymptomatic cases.

This first diagnosis provides information at a very early stage of the disease in order to quickly put in place the first preventive measures to avoid contagion.

Stage of development

TLR9 - Technology Readiness Level

TLR9 - Technology Readiness Level

Rapid detection of contagious bovine pleuropneumonia

The CFT test has several advantages, including:

- The detection of sick animals very early on, the detection time is shorter than for the cELISA;

- The possession of a sensitivity equivalent to the cELISA’s, the latter however remains much more specific (98% for the CFT vs. ≥99.9% for the cELISA);

- Being supplied as a kit containing approximately 1000 tests, with all the necessary reagents except red blood cells;

- Not requiring freezing: kit storage at +4°C;

- Being less expensive than the cELISA test.

The essential role of veterinary laboratories and centers in surveillance

This test, intended for national and regional laboratories and veterinary centers, allows the diagnosis of CBPP at the herd level: confirmation of outbreaks, surveillance and eradication plans.

In case of discovery of an outbreak of CBPP, laboratories and veterinarians should contact the local health authorities. On request, our experts will be able to support you in proposing recommendations to contain the expansion of the disease and to advice you on the diagnoses adapted to the context.

CIRAD offers technical training in CBPP diagnosis, in addition to technical training to support veterinary centers.

How to obtain the CFT diagnostic kits for CBPP?

The CFT PPCB diagnostic kit is distributed by CIRAD with a minimum order of 10 units (10 000 tests). The kits are delivered by express mail, within one week, after receipt of a firm order.

For prices and payment terms, please contact us.

The research team: The ASTRE research unit has about 100 staff and is the WHO reference laboratory for the following diseases: peste des petits ruminants, contagious bovine peripneumonia, contagious caprine peripneumonia, cowdriosis, rinderpest and, in association with the INTERTRYP research unit, diseases due to trypanosomatids (animal and human trypanosomoses, leishmaniasis). It is also the EU and FAO reference laboratory for PPR.

References and intellectual property

in addition to the IOE PPCB Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (also known as the Terrestrial Manual)

Publications

Amanfu W., Sediadie S., Masupu K.V., Benkirane A., Geiger R. & Thiaucourt F. (1998). Field validation of a competitive ELISA for the detection of contagious bovine pleuropneumonia in Botswana. Rev. Elev. Med. Vet. Pays Trop., 51, 189–193.

Bellini S., Giovannini A., Di Francesco C., Tittarelli M. & Caporale V. (1998). Sensitivity and specificity of serological and bacteriological tests for contagious bovine pleuropneumonia. Rev. sci. tech. OIE Int. Epiz., 17, 654–659.

CAMPBELL A.D., TURNER A.W., 1953. Studies on contagious bovine pleuropneumonia of cattle. IV. An improved complement fixation test. Aust. vet. J., 29: 154-163.

Niang M., Diallo M., Cisse O., Kone M., Doucoure M., Roth J.A., Balcer-Rodrigues V. & Dedieu L. (2006). Pulmonary and serum antibody responses elicited in zebu cattle experimentally infected with Mycoplasma mycoides subsp. mycoides SC by contact exposure. Vet. Res., 37, 733–744.

Provost A. (1972). Recherches immunologiques sur la péripneumonie. XIV. Description de deux techniques applicables sur le terrain pour le diagnostic de la maladie. Rev. Elev. Med. Vet. Pays Trop., 25, 475–496.

Provost A., Perreau P., Breard A., Le Goff C., Martel J.L. & Cottew G.S. (1987). Péripneumonie contagieuse bovine. Rev. sci. tech. Off. int. Epiz., 6, 565–624.