ELISA diagnostic kit to combat CBPP

Veterinary product Animal production and health
How can clusters of contagious bovine pleuropneumonia (CBPP) be contained? Serological diagnosis using a competitive ELISA (cELISA) is an effective way of combating CBPP!
Colonies de Mycoplasma mycoides subsp. mycoides biotype Small Colony. © F. Thiaucourt, Cirad
Colonies de Mycoplasma mycoides subsp. mycoides biotype Small Colony. © F. Thiaucourt, Cirad

Colonies of Mycoplasma mycoides subsp. mycoides  © F. Thiaucourt, CIRAD

Stemming the spread of CBPP

CBPP is a serious respiratory disease that threatens cattle farms, particularly in Africa. Because of the number of asymptomatic cases, surveillance of herds is essential to control the disease.

Developed by CIRAD, the world reference laboratory for PPCB for the FAO and the World Organization for Animal Health (WOAH), the ELISA PPCB test is a reference method for the detection of serum antibodies against this disease. Based on a monoclonal antibody, the ELISA is far more specific than complement fixation reaction (CFR) tests. Both tests are recommended by WHOA for the diagnosis of CBPP in herds. In particular, they are prescribed for outbreak confirmation, surveillance, eradication plans and confirmation of disease-free status prior to export.

Stage of development

TLR9 - Technology Readiness Level

TLR9 - Technology Readiness Level

Benefits of the ELISA diagnostic kit

Benefits of ELISA PPCB diagnostic kit vs. RFC test (IgM detection, earlier):

  • Higher specificity (≥99.9%) with equivalent sensitivity; vs. RFC Test (specificity, 98%; sensitivity 63.8%)
  • Detection of IgG antibodies, more persistent over time (detection of animals in chronic phase)
  • Excellent robustness, repeatability and reproducibility
  • Analysis simplification and automation: many serums can be processed in series for screening campaigns
  • Speed: results in less than 3 hours.
  • Marketed as a complete, ready-to-use kit (≥450 tests per kit with break-off strips)
  • No need for freezing: kit can be stored at +4°C

The essential role of veterinary laboratories and centers in surveillance

The ELISA PPCB test, intended for national and regional laboratories and veterinary centers, enables PPCB to be diagnosed in herds.

In the event of suspicion/discovery of an outbreak, veterinary services should contact local health authorities. On request, our experts can provide support and recommendations to contain the spread of the disease, and recommend diagnostics adapted to the context.

CIRAD offers technical training courses to support veterinary centers:

- Diagnostic techniques for CBPP 

How to obtain the PPCB ELISA diagnostic kit?

Order your kit from our partner Innovation Diagnostics, who markets the diagnostic kit under the name: ID Screen® CBPP Competition.

Contact your local representative on the Innovative Diagnostics website.

References and intellectual property: in addition to the WHOA "Terrestrial Manual" for CBPP 

Le Goff C., Thiaucourt F., 1998. A competitive ELISA for the specific diagnosis of contagious bovine pleuropneumonia (CBPP). Vet Microbiol 60: 179-191

Bellini S., Giovannini A., Di Francesco C., Tittarelli M. & Caporale V. (1998). Sensitivity and specificity of serological and bacteriological tests for contagious bovine pleuropneumonia. Rev. sci. tech. OIE Int. Epiz., 17, 654–659.

Amanfu W., Sediadie S., Masupu K.V., Benkirane A., Geiger R. & Thiaucourt F. (1998). Field validation of a competitive ELISA for the detection of contagious bovine pleuropneumonia in Botswana. Rev. Elev. Med. Vet. Pays Trop., 51, 189–193.

Niang M., Diallo M., Cisse O., Kone M., Doucoure M., Roth J.A., Balcer-Rodrigues V. & Dedieu L. (2006). Pulmonary and serum antibody responses elicited in zebu cattle experimentally infected with Mycoplasma mycoides subsp. mycoides SC by contact exposure. Vet. Res., 37, 733–744.